Journal: RSC Advances

Article Title: A multi-stage computational pipeline and in vitro validation for the discovery of small-molecule translation inhibitors targeting the bacterial ribosome

doi: 10.1039/d6ra01785a

Figure Lengend Snippet: Cartoon representation of E. coli 70S ribosome (PDB ID 4v7v), where small (30S) and large (50S) subunits are displayed with ribosomal proteins on the left. The investigated region, including the peptidyl transferase center on 23S rRNA, is indicated on 50S on the right. The native inhibitor Clindamycin (CLY) is shown in sticks. Mg 2+ ions in the crystal structure are depicted as magenta spheres.

Article Snippet: Among these candidates, only Mitoxantrone and Daunorubicin exhibited antibacterial activity against E. coli ATCC 10536 (Table S4–S6).

Techniques:

Journal: RSC Advances

Article Title: A multi-stage computational pipeline and in vitro validation for the discovery of small-molecule translation inhibitors targeting the bacterial ribosome

doi: 10.1039/d6ra01785a

Figure Lengend Snippet: In vitro inhibition of protein synthesis by Clindamycin (control) and the drug candidates in the E. coli cell-free translation system. Error bars represent standard error of the mean. Any error bars that are not visible are smaller than the data symbols.

Article Snippet: Among these candidates, only Mitoxantrone and Daunorubicin exhibited antibacterial activity against E. coli ATCC 10536 (Table S4–S6).

Techniques: In Vitro, Inhibition, Control

Journal: eBioMedicine

Article Title: A next-generation probiotic strain for gut health: Bacteroides cellulosilyticus LYH2 variant with anti-inflammatory and metabolic advantages

doi: 10.1016/j.ebiom.2026.106232

Figure Lengend Snippet: Metabolite profile of B. cellulosilyticus LYH2 and demonstration of the antimicrobial potential of its metabolites in co-cultivation with pathogenic bacteria. (A) Production of SCFAs by B. cellulosilyticus LYH2 cultured in BHI medium. Quantified SCFAs include AA, PA, BA, IBA, VA, and IVA. Data are presented as mean ± SEM. Asterisks denote statistical significance (∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001) as determined by unpaired two-tailed Student's t -test (∗ P < 0.05). Isovalerate was detected exclusively in the B. cellulosilyticus LYH2 group and was below the limit of detection in the BHI control. Therefore, no statistical comparison was applicable. (B–C) The volcano plots visually represent the magnitude fold change (|log2FC| > 1) and statistical significance (FDR < 0.05) of metabolites in positive and negative ion modes, contrasting their abundance in Ce and BHI media (n = 3). (D–E) The Principal Component Analysis (PCA) plots depict the clustering of samples based on positive and negative ion mode metabolite profiles in BHI and Ce media. Distinct colours represent separate groups. (F) A bar plot presents the enrichment of KEGG pathways, comparing the metabolic differences between Ce and BHI media (n = 3). (G) The preparation and subsequent co-culture process of B. cellulosilyticus LYH2 metabolites. (i) Metabolite solids are obtained via nitrogen evaporation under high-purity nitrogen conditions. (ii) Comparison of bacterial culture concentrations before and after the addition of B. cellulosilyticus LYH2 metabolites. The tubes on the left and right serve as positive controls containing pathogens, while the middle tubes represent the metabolite treatment group. (H–J) Growth curves of E. coli , S. aureus , and S. T yphimurium with and without the addition of metabolites (n = 3).

Article Snippet: Antibacterial activity against E. coli ATCC 25922, S. aureus ATCC 25923, and S. T yphimurium ATCC 14028 was assessed by co-culturing, with detailed methods provided in the .

Techniques: Bacteria, Cell Culture, Two Tailed Test, Control, Comparison, Co-Culture Assay, Evaporation